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Changes in T-Cell and Monocyte PhenotypesIn VitrobySchistosoma mansoniAntigens in Cutaneous Leishmaniasis Patients
- Source :
- Journal of Parasitology Research, Journal of Parasitology Research, Vol 2012 (2012)
- Publication Year :
- 2012
- Publisher :
- Hindawi Limited, 2012.
-
Abstract
- High levels of proinflammatory cytokines such as IFN-γand TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated thatSchistosoma mansoniantigens downmodulate thein vitrocytokine response in CL. In the current study we evaluated whetherS. mansoniantigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured withL. braziliensisantigen in the presence or absence of theS. mansoniantigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14+CD16++) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14++CD16-) and intermediate (CD14++CD16+) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4+T cells and they also expanded the frequency of CD4+CD25highFoxp3+T cells. Taken together, we show thatS. mansoniantigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes.
- Subjects :
- Article Subject
biology
T cell
CD14
Monocyte
biology.organism_classification
Molecular biology
Peripheral blood mononuclear cell
lcsh:Infectious and parasitic diseases
Infectious Diseases
medicine.anatomical_structure
Antigen
Immunology
Clinical Study
medicine
lcsh:RC109-216
Parasitology
Schistosoma mansoni
IL-2 receptor
CD80
Subjects
Details
- ISSN :
- 20900031 and 20900023
- Volume :
- 2012
- Database :
- OpenAIRE
- Journal :
- Journal of Parasitology Research
- Accession number :
- edsair.doi.dedup.....175363405a6df023b7bcf79f25c9d628