Supplementary Figure 1 from PML/RARα-Regulated miR-181a/b Cluster Targets the Tumor Suppressor RASSF1A in Acute Promyelocytic Leukemia

A, Apoptosis assay in NB4 cells 24h and 48h after application of As2O3 (1 µM). B, FACS analysis for CD11b expression in As2O3 treated NB4 cells 48h after application. C, qPCR analysis for C/EBPβ mRNA expression in NB4 cells after As2O3 application. D, Apoptosis assay in NB4 cells 48h after application of doxorubicin (0.01 µg/ml) with or without cytarabine (0.05 µM). E, qPCR analys for C/EBPβ mRNA expression in NB4 cells after doxorubicin and cytarabine application. For qPCR measurement GAPDH was used as control. Bars and values in the dot blots represent the median {plus minus} SD from three independent experiments.* p-value {less than or equal to} 0.05; ** p-value {less than or equal to} 0.01.