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Electromagnetic Field (EMF) Radiation Alters Estrogen Release from the Pig Myometrium during the Peri-Implantation Period
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 2920, p 2920 (2021), International Journal of Molecular Sciences, Volume 22, Issue 6
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- An electromagnetic field (EMF) may affect the functions of uterine tissues. This study hypothesized that EMF changes the estrogenic activity of pig myometrium during the peri-implantation period. Tissue was collected on days 15–16 of the gestation and incubated in the presence of EMF (50 and 120 Hz, 2 and 4 h). The cytochrome P450 aromatase type 3 (CYP19A3) and hydroxysteroid 17β dehydrogenase type 4 (HSD17B4) mRNA transcript abundance, cytochrome P450arom (aromatase), and 17β hydroxysteroid dehydrogenase 17βHSD) protein abundance and estrone (E1) and estradiol-17β (E2) release were examined using Real-Time PCR, Western blot and radioimmunoassay. Selected myometrial slices were treated with progesterone (P4) to determine whether it functions as a protector against EMF. CYP19A3 mRNA transcript abundance in slices treated with EMF was less at 50 Hz (2 h) and greater at 120 Hz (2 and 4 h). HSD17B4 mRNA transcript was greater in slices treated with EMF at 120 Hz (2 h). Progesterone diminished EMF-related effects on CYP19A3 and HSD17B4. When P4 was added, EMF had suppressive (50 and 120 Hz, 2 h) or enhancing (50 Hz, 4 h) effects on aromatase abundance. The E1 release was lower after 4 h of EMF treatment at 50 Hz and P4 did not protect myometrial E1 release. In conclusion, EMF alters the synthesis and release of E1 and did not affect E2 release in the myometrium during the peri-implantation period.
- Subjects :
- 0301 basic medicine
steroidogenesis
Swine
17βHSD
Tissue Culture Techniques
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
Aromatase
Hydroxysteroid dehydrogenase
Peroxisomal Multifunctional Protein-2
lcsh:QH301-705.5
Progesterone
Spectroscopy
030219 obstetrics & reproductive medicine
Estradiol
biology
medicine.diagnostic_test
Electromagnetic Radiation
myometrium
Myometrium
pigs
Radioimmunoassay
General Medicine
Computer Science Applications
Female
Hydroxysteroid
estrogens
aromatase
animal structures
Estrone
medicine.drug_class
Article
Catalysis
Inorganic Chemistry
Andrology
03 medical and health sciences
Electromagnetic Fields
Western blot
electromagnetic field
medicine
Animals
Embryo Implantation
RNA, Messenger
Physical and Theoretical Chemistry
Molecular Biology
uterus
Organic Chemistry
030104 developmental biology
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Estrogen
biology.protein
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....173fe0227e0fa6a0ad9a84bd91470fb9