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Standardized thionin-eosin Y: a quick stain for cytology
- Source :
- Stain technology. 64(5)
- Publication Year :
- 1989
-
Abstract
- Two stains long used in exfoliative cytology, the hematoxylin-eosin Y and Papanicolaou stains, have not been standardized even today. Some dozens of hematoxylin and eosin and Papanicolaou staining recipes have been recommended in the literature. Consequently, the staining pattern of hematoxylin and eosin, and Papanicolaou stained cytological material varies from laboratory to laboratory. To a certain degree this is due to batch-to-batch variations of commercial samples of the natural dye hematoxylin (C.I. 75290). The present paper describes a simple, standardized and reproducible procedure using thionin bromide to replace hematoxylin in the hematoxylin and eosin stain.
- Subjects :
- Pathology
medicine.medical_specialty
Lung Neoplasms
integumentary system
Staining and Labeling
business.industry
animal diseases
Histological Techniques
H&E stain
Papanicolaou stain
Adenocarcinoma
Stain
Staining
Thionin
chemistry.chemical_compound
chemistry
Phenothiazines
Cytology
Medicine
Eosine Yellowish-(YS)
Humans
Anatomy
Exfoliative cytology
business
Eosin Y
Subjects
Details
- ISSN :
- 00389153
- Volume :
- 64
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Stain technology
- Accession number :
- edsair.doi.dedup.....173cdaee7ee0a2d3e202b52c6b6955c8