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Decreased blood riboflavin levels are correlated with defective expression ofRFT2gene in gastric cancer
- Source :
- World Journal of Gastroenterology. 18:3112
- Publication Year :
- 2012
- Publisher :
- Baishideng Publishing Group Inc., 2012.
-
Abstract
- AIM: To investigate the relationship between blood riboflavin levels and riboflavin transporter 2 (RFT2) gene expression in gastric carcinoma (GC) development. METHODS: High-performance liquid chromatography was used to detect blood riboflavin levels in patients with GC. Real-time fluorogenic quantitative polymerase chain reaction and immunohistochemistry were used to analyze the expression of RFT2 mRNA and protein in samples from 60 GC patients consisting of both tumor and normal tissue. RESULTS: A significant decrease in the RFT2 mRNA levels was detected in GC samples compared with those in the normal mucous membrane (0.398 ± 0.149 vs 1.479 ± 0.587; P = 0.040). Tumors exhibited low RFT2 protein expression (75%, 16.7%, 8.3% and 0% for no RFT2 staining, weak staining, medium staining and strong staining, respectively), which was significantly lower than that in the normal mucous membrane (10%, 16.7%, 26.7% and 46.7% for no RFT2 staining, weak staining, medium staining and strong staining, respectively; P < 0.05). Tumors with low RFT2 expression were significantly associated with tumor stage and histological grade. Moreover, a significantly decrease in Uyghur patients was observed compared with Han patients. However, other parameters-gender, tumor location and lymph node metastasis-showed no significant relationship with RFT2 expression. Blood riboflavin levels were reverse correlated with development of GC (1.2000 ± 0.97 569 ng/mL in high tumor stage patients vs 2.5980 ± 1.31 129 ng/mL in low tumor stage patients; P < 0.05). A positive correlation of plasma riboflavin levels with defective expression of RFT2 protein was found in GC patients (χ2 = 2.619; P = 0.019). CONCLUSION: Defective expression of RFT2 is associated with the development of GC and this may represent a mechanism underlying the decreased plasma riboflavin levels in GC.
- Subjects :
- Adult
Male
China
Brief Article
Riboflavin
Down-Regulation
Real-Time Polymerase Chain Reaction
Asian People
Stomach Neoplasms
Gene expression
medicine
Humans
heterocyclic compounds
RNA, Messenger
Lymph node
Chromatography, High Pressure Liquid
Aged
Neoplasm Staging
Chi-Square Distribution
biology
Reverse Transcriptase Polymerase Chain Reaction
Membrane transport protein
digestive, oral, and skin physiology
Gastroenterology
food and beverages
Membrane Transport Proteins
Mucous membrane
Cell Differentiation
General Medicine
Middle Aged
Immunohistochemistry
Molecular biology
Staining
Gene Expression Regulation, Neoplastic
Real-time polymerase chain reaction
medicine.anatomical_structure
Lymphatic Metastasis
biology.protein
Female
human activities
Subjects
Details
- ISSN :
- 10079327
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- World Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....171774fa68e7908b826054c515eac013
- Full Text :
- https://doi.org/10.3748/wjg.v18.i24.3112