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Expression of Cry1Ac toxin-binding region in Plutella xyllostella cadherin-like receptor and studying their interaction mode by molecular docking and site-directed mutagenesis
- Source :
- International Journal of Biological Macromolecules. 111:822-831
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Cadherin-like protein has been identified as the primary Bacillus thuringiensis (Bt) Cry toxin receptor in Lepidoptera pests and plays a key role in Cry toxin insecticidal. In this study, we successfully expressed the putative Cry1Ac toxin-binding region (CR7–CR11) of Plutella xylostella cadherin-like in Escherichia coli BL21 (DE3). The expressed CR7–CR11 fragment showed binding ability to Cry1Ac toxin under denaturing (Ligand blot) and non-denaturing (ELISA) conditions. The three-dimensional structure of CR7–CR11 was constructed by homology modeling. Molecular docking results of CR7–CR11 and Cry1Ac showed that domain II and domain III of Cry1Ac were taking part in binding to CR7–CR11, while CR7–CR8 was the region of CR7–CR11 in interacting with Cry1Ac. The interaction of toxin-receptor complex was found to arise from hydrogen bond and hydrophobic interaction. Through the computer-aided alanine mutation scanning, amino acid residues of Cry1Ac (Met341, Asn442 and Ser486) and CR7–CR11 (Asp32, Arg101 and Arg127) were predicted as the hot spot residues involved in the interaction of the toxin-receptor complex. At last, we verified the importance role of these key amino acid residues by binding assay. These results will lay a foundation for further elucidating the insecticidal mechanism of Cry toxin and enhancing Cry toxin insecticidal activity by molecular modification.
- Subjects :
- 0301 basic medicine
Insecticides
Bacillus thuringiensis
medicine.disease_cause
Biochemistry
Hemolysin Proteins
03 medical and health sciences
Bacterial Proteins
Structural Biology
medicine
Animals
Amino Acid Sequence
Homology modeling
Site-directed mutagenesis
Molecular Biology
Escherichia coli
Alanine
Bacillus thuringiensis Toxins
030102 biochemistry & molecular biology
biology
Toxin
Chemistry
Ligand binding assay
fungi
General Medicine
Cadherins
biology.organism_classification
Endotoxins
Lepidoptera
Molecular Docking Simulation
030104 developmental biology
Cry1Ac
Larva
Mutation
Mutagenesis, Site-Directed
Protein Binding
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 111
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....171449455840af1375998350c2c17f88