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B-vitamins intake, DNA-methylation of One Carbon Metabolism and homocysteine pathway genes and myocardial infarction risk: The EPICOR study
- Source :
- Nutrition, Metabolism and Cardiovascular Diseases. 24:483-488
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Background and aims Several epidemiological studies highlighted the association between folate and B-vitamins low intake and cardiovascular diseases (CVD) risk. Contrasting results were reported on the relationship between folate intake and DNA-methylation. Folate and B-vitamins may modulate DNA-methylation of specific enzymes which are included in the One-Carbon Metabolism (OCM) and in the homocysteine (Hcy) pathways. The aim of the study was to evaluate whether DNA-methylation profiles of OCM and Hcy genes could modulate the myocardial infarction (MI) risk conferred by a low B-vitamins intake. Methods and results Study sample (206 MI cases and 206 matched controls) is a case-control study nested in the prospective EPIC cohort. Methylation levels of 33 candidate genes where extracted by the whole epigenome analysis (Illumina-HumanMethylation450K-BeadChip). We identified three differentially methylated regions in males (TCN2 promoter, CBS 5′UTR, AMT gene-body) and two in females (PON1 gene-body, CBS 5′UTR), each of them characterized by an increased methylation in cases. Functional in silico analysis suggested a decreased expression in cases. A Recursively Partitioned Mixture Model cluster algorithm identified distinct methylation profiles associated to different MI risk: high-risk vs. low-risk methylation profile groups, OR = 3.49, p = 1.87 × 10 − 4 and OR = 3.94, p = 0.0317 in males and females respectively (multivariate logistic regression adjusted for classical CVD risk factors). Moreover, a general inverse relationship between B-vitamins intake and DNA-methylation of the candidate genes was observed. Conclusions Our findings support the hypothesis that DNA-methylation patterns in specific regions of OCM and Hcy pathways genes may modulate the CVD risk conferred by folate and B-vitamins low intake.
- Subjects :
- Adult
Male
Candidate gene
medicine.medical_specialty
B-vitamins
DNA-methylation
Homocysteine
Myocardial Infarction
One Carbon Metabolism
Endocrinology, Diabetes and Metabolism
Medicine (miscellaneous)
Biology
chemistry.chemical_compound
Risk Factors
Internal medicine
medicine
Aminomethyltransferase
Humans
Prospective Studies
Promoter Regions, Genetic
Genetics
Transcobalamins
Nutrition and Dietetics
Aryldialkylphosphatase
Methylation
Epigenome
DNA Methylation
Middle Aged
PON1
B vitamins
Logistic Models
Differentially methylated regions
Endocrinology
chemistry
Case-Control Studies
Multivariate Analysis
Vitamin B Complex
DNA methylation
Female
Cardiology and Cardiovascular Medicine
Metabolic Networks and Pathways
Follow-Up Studies
Subjects
Details
- ISSN :
- 09394753
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Nutrition, Metabolism and Cardiovascular Diseases
- Accession number :
- edsair.doi.dedup.....17118896a79400de7b5d0468a5ea8500