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Functional region identification in proteins by accumulative-quantitative peptide mapping using RP-HPLC-MS
- Source :
- Journal of Agricultural and Food Chemistry 55 (2007) 23, Journal of Agricultural and Food Chemistry, 55(23), 9337-9344
- Publication Year :
- 2007
-
Abstract
- A new method was developed to identify regions in proteins from which peptides are derived with specific functional properties. This method is applicable for systems in which peptides of a hydrolyzed protein possess specific functional properties, but are too large to be sequenced directly and/or the peptide mixture is too complex to purify and characterize each peptide individually. In the present work, aggregating peptides obtained by proteolytic hydrolysis of soy glycinin were used as a case study. The aggregating peptides are isolated and subsequently further degraded with trypsin to result in peptides with a mass
- Subjects :
- Hydrolyzed protein
Proteolysis
Ion chromatography
Peptide
Chemical Fractionation
Mass spectrometry
High-performance liquid chromatography
Peptide Mapping
Mass Spectrometry
Levensmiddelenchemie
medicine
Amino Acid Sequence
acids
Chromatography, High Pressure Liquid
VLAG
chemistry.chemical_classification
Chromatography
Food Chemistry
medicine.diagnostic_test
Chemistry
Hydrolysis
Globulins
General Chemistry
mass-spectrometry
Trypsin
soy proteins
Biochemistry
Soybean Proteins
Bottom-up proteomics
General Agricultural and Biological Sciences
Peptides
absorption
allergen
medicine.drug
Subjects
Details
- ISSN :
- 00218561
- Volume :
- 55
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Journal of agricultural and food chemistry
- Accession number :
- edsair.doi.dedup.....170ccb98192c53d1fa373a65f78fe658