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Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene
- Source :
- Acta Veterinaria Hungarica. 61:1-8
- Publication Year :
- 2013
- Publisher :
- Akademiai Kiado Zrt., 2013.
-
Abstract
- The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.
- Subjects :
- DNA, Bacterial
Serotype
education.field_of_study
Pasteurella multocida
General Veterinary
Pasteurella Infections
Population
Biology
HindIII
biology.organism_classification
Polymerase Chain Reaction
law.invention
Microbiology
Birds
Restriction enzyme
law
Polymorphism (computer science)
biology.protein
Animals
Restriction fragment length polymorphism
education
Polymorphism, Restriction Fragment Length
Polymerase chain reaction
Subjects
Details
- ISSN :
- 15882705 and 02366290
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Acta Veterinaria Hungarica
- Accession number :
- edsair.doi.dedup.....170c1f42c89e8de02024871f08dc7eb6