Back to Search
Start Over
HaCaT keratinocytes exhibit a cholesterol and plasma membrane viscosity gradient during directed migration
- Source :
- Experimental Cell Research. 318:809-818
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Keratinocyte migration plays an important role in cutaneous wound healing by supporting the process of reepithelialisation. During directional migration cells develop a polarised shape with an asymmetric distribution of a variety of signalling molecules in their plasma membrane. Here, we investigated front-to-back differences of the physical properties of the plasma membrane of migrating keratinocyte-like HaCaT cells. Using FRAP and fluorescence lifetime analysis, both under TIR illumination, we demonstrate a reduced viscosity of the plasma membrane in the lamellipodia of migrating HaCaT cells compared with the cell rears. This asymmetry is most likely caused by a reduced cholesterol content of the lamellipodia as demonstrated by filipin staining. siRNA-mediated silencing of the cholesterol transporter ABCA1, which is known to redistribute cholesterol from rafts to non-raft regions, as well as pharmacological inhibition of this transporter with glibenclamide, strongly diminished the viscosity gradient of the plasma membrane. In addition, HaCaT cell migration was inhibited by glibenclamide treatment. These data suggest a preferential role of non-raft cholesterol in the establishment of the asymmetric plasma membrane viscosity.
- Subjects :
- Keratinocytes
Biology
Filipin
chemistry.chemical_compound
Membrane Microdomains
Cell Movement
Glyburide
Humans
Hypoglycemic Agents
Gene Silencing
Pseudopodia
Keratinocyte migration
Reduced viscosity
Cells, Cultured
Viscosity
Cell Membrane
Cell migration
Cell Biology
Cell biology
HaCaT
Cholesterol
Membrane
chemistry
ABCA1
biology.protein
ATP-Binding Cassette Transporters
lipids (amino acids, peptides, and proteins)
Lamellipodium
ATP Binding Cassette Transporter 1
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 318
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research
- Accession number :
- edsair.doi.dedup.....16fa159e4c9e461d90125320a57a6fbe