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Microdissection to Isolate Vascular Cambium Cells in Poplar
- Source :
- Silva Fennica, Silva Fennica, Suomen Metsätieteellinen Seura ry, 2012, 46 (1), pp.5-16, Silva Fennica, Vol 46, Iss 1 (2012), HAL, Silva Fennica 1 (46), 5-16. (2012), Scopus-Elsevier
- Publication Year :
- 2012
- Publisher :
- HAL CCSD, 2012.
-
Abstract
- Vascular cambium is the lateral meristem producing xylem cells inwards and phloem cells outwards in plant stem. Thus, in trees, the quality and quantity of wood is a result of highly regulated developmental process depending initially on the vascular cambium cell production. The availability of accurate transcriptomics technologies based on high coverage sequencing raises the level of expectations on tissue sampling to a very high degree. What is the benefit of top-level transcriptomics in wood formation studies if we are using these technologies on raw tissues, mixing cells at the organ level or even higher scale? The presented work describes a nine-step procedure, from standing tree to isolated ray and fusiform cells from cryolyophilized tangential sections of the poplar cambial zone. The aim of this paper is to present a step by step procedure including advices on how to select the optimal tree, how to fell the tree while securing its physiological parameters, how to cryolyophilize and microdissect under binocular, presenting the time schedule of the whole process and RNA analysis.
- Subjects :
- 0106 biological sciences
fusiform cambial cells
microgenomics
Populus spp
ray cambial cells
[SDV]Life Sciences [q-bio]
populus
Biology
faisceau vasculaire
01 natural sciences
Transcriptome
03 medical and health sciences
Vascular cambium
lcsh:Forestry
Process (anatomy)
Microdissection
030304 developmental biology
Plant stem
0303 health sciences
xylème
Ecological Modeling
fungi
Xylem
Forestry
15. Life on land
Meristem
Cell biology
lcsh:SD1-669.5
Phloem
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 00375330
- Database :
- OpenAIRE
- Journal :
- Silva Fennica, Silva Fennica, Suomen Metsätieteellinen Seura ry, 2012, 46 (1), pp.5-16, Silva Fennica, Vol 46, Iss 1 (2012), HAL, Silva Fennica 1 (46), 5-16. (2012), Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....16f6e54cc58024682cff4a4ebcc8ca15