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Effect of Storage Temperature on the Stability of Spray Dried Bacteriophage Powders
- Publication Year :
- 2018
-
Abstract
- This study aimed to assess the robustness of using a spray drying approach and formulation design in producing inhalable phage powders. Two types of Pseudomonas phages, PEV2 (Podovirus) and PEV40 (Myovirus) in two formulations containing different amounts of trehalose (70% and 60%) and leucine (30% and 40%) were studied. Most of the surface of the produced powders was found to be covered in crystalline leucine. The powders were stored at 4 °C and 20 °C under vacuum. The phage stability and in vitro aerosol performance of the phage powders were examined on the day of production and after 1, 3 and 12 months of storage. A minor titer loss during production was observed for both phages (0.2–0.8 log10 pfu/ml). The storage stability of the produced phage powders was found to be phage and formulation dependent. No further reduction in titer occurred for PEV2 powders stored at 4 °C across the study. The formulation containing 30% leucine maintained the viability of PEV2 at 20 °C, while the formulation containing 40% leucine gradually lost titer over time with a storage reduction of ∼0.9 log10 pfu/ml measured after 12 months. In comparison, the PEV40 phage powders generally had a ∼ 0.5 log10 pfu/ml loss upon storage regardless of temperature. When aerosolized, the total in vitro lung doses of PEV2 were of the order of 107 pfu, except the formulation containing 40% leucine stored at 20 °C which had a lower lung dose. The PEV40 powders also had lung doses of 106–107 pfu. The results demonstrate that spray dried Myoviridae and Podoviridae phage in a simple formulation of leucine and trehalose can be successfully stored for one year at 4 °C and 20 °C with vacuum packaging. The University of Sydney; Australian Research Council; National Institute of Allergy and Infectious Diseases of the National Institutes of Health; National Health and Medical Research Council Centre of Research Excellence in Tuberculosis Control
- Subjects :
- 0301 basic medicine
Antibiotic resistance
viruses
Chemistry, Pharmaceutical
090406 - Powder and Particle Technology [FoR]
Pharmaceutical Science
02 engineering and technology
Vacuum packing
111504 - Pharmaceutical Sciences [FoR]
Article
Bacteriophage
Excipients
03 medical and health sciences
chemistry.chemical_compound
Drug Stability
Administration, Inhalation
Bacteriophages
Desiccation
Particle Size
Lung
Aerosolization
Aerosols
Chromatography
PEV40
biology
Temperature
Trehalose
Dry Powder Inhalers
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
PEV2
3. Good health
Titer
030104 developmental biology
chemistry
110203 - Respiratory Diseases [FoR]
110309 - Infectious Diseases [FoR]
Spray drying
Phage
Particle size
Leucine
Powders
0210 nano-technology
Pulmonary infections
Phage dry powder
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....16ef0b7662b7fadc73dc9bb640f02f7f