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Glomerular parietal epithelial cell activation induces collagen secretion and thickening of Bowman's capsule in diabetes
- Source :
- Laboratory Investigation. 95:273-282
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- The metabolic and hemodynamic alterations in diabetes activate podocytes to increase extracellular matrix (ECM) production, leading to thickening of the glomerular basement membrane (GBM). We hypothesized that diabetes would activate parietal epithelial cells (PECs) in a similar manner and cause thickening of Bowman’s capsules. Periodic acid Schiff staining of human kidney biopsies of 30 patients with diabetic nephropathy (DN) revealed a significantly thicker Bowman’s capsule as compared with 20 non-diabetic controls. The average thickness was 4.55±0.21 μm in the group of patients with DN compared with 2.92±0.21 μm in the group of non-diabetic controls (P
- Subjects :
- Adult
Glycation End Products, Advanced
Male
medicine.medical_specialty
Bowman's capsule
Blotting, Western
Kidney Glomerulus
Gene Expression
Periodic acid–Schiff stain
Perlecan
Pathology and Forensic Medicine
Transforming Growth Factor beta1
Extracellular matrix
Diabetic nephropathy
Microscopy, Electron, Transmission
Laminin
Internal medicine
medicine
Humans
Diabetic Nephropathies
Molecular Biology
Cells, Cultured
biology
Reverse Transcriptase Polymerase Chain Reaction
Glomerular basement membrane
Epithelial Cells
Bowman Capsule
Cell Biology
medicine.disease
Immunohistochemistry
Epithelium
Collagen Type I, alpha 1 Chain
Glucose
Hyaluronan Receptors
Endocrinology
medicine.anatomical_structure
biology.protein
Female
Collagen
Subjects
Details
- ISSN :
- 00236837
- Volume :
- 95
- Database :
- OpenAIRE
- Journal :
- Laboratory Investigation
- Accession number :
- edsair.doi.dedup.....16d6f99ca691eb7156fabba065cb78e9