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The regulatory role of ProBDNF in monocyte function: Implications in Stanford type-A aortic dissection disease
- Source :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 34(2)
- Publication Year :
- 2019
-
Abstract
- Brain-derived neurotrophic factor precursor (proBDNF) has been reported to strengthen the dysfunction of monocytes/macrophages in animal studies. However,it is still unknown the roles of proBDNF in the dysfunction of monocytes in the inflammatory diseases in humans. In the present study, we showed that proBDNFand pan neurotrophic receptor p75 were significantly upregulated in monocytes from healthy donors (HD) after lipopolysaccharide treatment. Exogenous proBDNF treatment upregulated CD40 and proinflammatory cytokines expression in monocytes including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. InStanford type-A acute aortic dissection (AAD) patients, proBDNF was upregulatedin CD14+CD163+CX3CR1+ M2- but not CD14+CD68+CCR2+M1-like monocytes.In addition, sera from AAD patients activated gene expression of proinflammatory cytokines in cultured PBMCs from HD, which was attenuated by proBDNF monoclonal antibody (Ab-proB) treatment. These findings suggested that upregulation ofproBDNF in M2-like monocytes may contribute to the proinflammatory response in the AAD. Refereed/Peer-reviewed
- Subjects :
- 0301 basic medicine
Adult
Male
CD14
Biochemistry
Peripheral blood mononuclear cell
Monocytes
Proinflammatory cytokine
03 medical and health sciences
0302 clinical medicine
Neurotrophic factors
Genetics
medicine
Humans
Protein Precursors
Molecular Biology
Cells, Cultured
CD68
business.industry
Tumor Necrosis Factor-alpha
Monocyte
Brain-Derived Neurotrophic Factor
Macrophages
Interleukin
Middle Aged
inflammatory disease
Up-Regulation
proBDNF
Aortic Dissection
030104 developmental biology
medicine.anatomical_structure
Stanford type-A acute aortic dissection
Immunology
Cytokines
Tumor necrosis factor alpha
Female
business
monocytes
030217 neurology & neurosurgery
Biotechnology
Subjects
Details
- ISSN :
- 15306860
- Volume :
- 34
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES
- Accession number :
- edsair.doi.dedup.....16b86dd9668cb276d95c18dfa3c9b1d1