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Highly Efficient Supramolecular Aggregation-Induced Emission-Active Pseudorotaxane Luminogen for Functional Bioimaging
- Source :
- Biomacromolecules. 18:886-897
- Publication Year :
- 2017
- Publisher :
- American Chemical Society (ACS), 2017.
-
Abstract
- The direct tracking of cells using fluorescent dyes is a constant challenge in cell therapy due to aggregation-induced quenching (ACQ) effect and biocompatibility issues. Here, we demonstrate the development of a biocompatible and highly efficient aggregation-induced emission (AIE)-active pseudorotaxane luminogen based on tetraphenylethene conjugated poly(ethylene glycol) (TPE-PEG2) (guest) and α-cyclodextrin (α-CD) (host). It is capable of showing significant fluorescent emission enhancement at the 400-600 nm range when excited at 388 nm, without increasing the concentration of AIE compound. The fluorescent intensity of TPE-PEG2 solution was effectively enhanced by 4-12 times with gradual addition of 1-4 mM of α-CD. 2D NOSEY 1H NMR revealed clear correlation spots between the characteristic peaks of α-CD and PEG, indicating the interaction between protons of ethylene glycol and cyclodextrin, and the structures are mainly based on threaded α-CD. The host-guest complex exhibits boosted fluorescent emission because the PEG side chains are confined in "nano-cavities" (host), thus, applying additional restriction on intermolecular rotation of TPE segments. In vitro cell experiments demonstrated the potential of AIE-active pseudorotaxane polymer as a biocompatible bioimaging probe. ASTAR (Agency for Sci., Tech. and Research, S’pore) MOE (Min. of Education, S’pore) Accepted version
- Subjects :
- alpha-Cyclodextrins
Aggregation-induced emissions
Magnetic Resonance Spectroscopy
Rotaxanes
Polymers and Plastics
Cell Survival
Polymers
alpha-Cyclodextrin
Supramolecular chemistry
Biocompatible Materials
Bioengineering
02 engineering and technology
Conjugated system
010402 general chemistry
Photochemistry
01 natural sciences
Micelle
Polyethylene Glycols
Biomaterials
chemistry.chemical_compound
Host guest complexes
Image Processing, Computer-Assisted
Materials Chemistry
Humans
Organic chemistry
Micelles
Fluorescent Dyes
chemistry.chemical_classification
Quenching (fluorescence)
Cyclodextrin
Optical Imaging
Epithelial Cells
Hep G2 Cells
021001 nanoscience & nanotechnology
Fluorescence
0104 chemical sciences
chemistry
A549 Cells
0210 nano-technology
Ethylene glycol
Subjects
Details
- ISSN :
- 15264602 and 15257797
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Biomacromolecules
- Accession number :
- edsair.doi.dedup.....16b12e694495ad540df5f9e5a85c64cd