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The Brain-Enriched MicroRNA miR-9-3p Regulates Synaptic Plasticity and Memory
- Publication Year :
- 2016
- Publisher :
- Society for Neuroscience, 2016.
-
Abstract
- MicroRNAs (miRNAs) are small, noncoding RNAs that posttranscriptionally regulate gene expression in many tissues. Although a number of brain-enriched miRNAs have been identified, only a few specific miRNAs have been revealed as critical regulators of synaptic plasticity, learning, and memory. miR-9-5p/3p are brain-enriched miRNAs known to regulate development and their changes have been implicated in several neurological disorders, yet their role in mature neurons in mice is largely unknown. Here, we report that inhibition of miR-9-3p, but not miR-9-5p, impaired hippocampal long-term potentiation (LTP) without affecting basal synaptic transmission. Moreover, inhibition of miR-9-3p in the hippocampus resulted in learning and memory deficits. Furthermore, miR-9-3p inhibition increased the expression of the LTP-related genes Dmd and SAP97, the expression levels of which are negatively correlated with LTP. These results suggest that miR-9-3p-mediated gene regulation plays important roles in synaptic plasticity and hippocampus-dependent memory. SIGNIFICANCE STATEMENT Despite the abundant expression of the brain-specific microRNA miR-9-5p/3p in both proliferating and postmitotic neurons, most functional studies have focused on their role in neuronal development. Here, we examined the role of miR-9-5p/3p in adult brain and found that miR-9-3p, but not miR-9-5p, has a critical role in hippocampal synaptic plasticity and memory. Moreover, we identified in vivo binding targets of miR-9-3p that are involved in the regulation of long-term potentiation. Our study provides the very first evidence for the critical role of miR-9-3p in synaptic plasticity and memory in the adult mouse.
- Subjects :
- 0301 basic medicine
Male
Receptors, CXCR4
Green Fluorescent Proteins
Nonsynaptic plasticity
Biology
Hippocampus
Discs Large Homolog 1 Protein
Dystrophin
03 medical and health sciences
Mice
Transduction, Genetic
Metaplasticity
Conditioning, Psychological
Animals
Humans
Maze Learning
Neuronal memory allocation
Neuronal Plasticity
General Neuroscience
Membrane Proteins
Long-term potentiation
Recognition, Psychology
Synapsin
Articles
Fear
Synapsins
Mice, Inbred C57BL
MicroRNAs
030104 developmental biology
Synaptic fatigue
HEK293 Cells
Synaptic plasticity
Exploratory Behavior
Memory consolidation
Neuroscience
Guanylate Kinases
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....16b07c2a2926e63781b601e85d1a6da2