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Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean
- Source :
- BMC Plant Biology, Vol 10, Iss 1, p 41 (2010), BMC Plant Biology
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- Background The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content. Results A soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix® Soy GeneChip and high-throughput Illumina® whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region. Conclusions This study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs and provide genome-wide transcriptome insight towards understanding seed biology and the soybean genome.
- Subjects :
- DNA, Plant
Sequence analysis
Quantitative Trait Loci
Genomics
Plant Science
Quantitative trait locus
Biology
Genome
Genetic analysis
Gene mapping
lcsh:Botany
Research article
Genomic Segment
Plant Oils
Gene
Oligonucleotide Array Sequence Analysis
Genetics
Polymorphism, Genetic
Gene Expression Profiling
Seed Storage Proteins
food and beverages
Sequence Analysis, DNA
Physical Chromosome Mapping
lcsh:QK1-989
Seeds
Soybeans
Genome, Plant
Subjects
Details
- ISSN :
- 14712229
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology
- Accession number :
- edsair.doi.dedup.....16aa14ff5446b3a1e643c9fea8a85d76