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Viability, proliferation and adhesion of smooth muscle cells and human umbilical vein endothelial cells on electrospun polymer scaffolds
- Source :
- Clinical Hemorheology and Microcirculation. 50:101-112
- Publication Year :
- 2012
- Publisher :
- IOS Press, 2012.
-
Abstract
- A major clinical problem of high relevance in the cardiovascular field is late stent thrombosis after implantation of drug eluting stents (DES). Clinical widely used DES currently utilize durable polymer coatings, which can induce persistent arterial wall inflammation and delayed vascular healing resulting in an impaired endothelialization. In this study we explored the interaction of smooth muscle cells (SMC) and human umbilical vein endothelial cells (HUVEC) with electrospun scaffolds prepared from resorbable polyetheresterurethane (PDC) and poly(p-dioxanone) (PPDO), as well as polyetherimide (PEI), which can be surface modified, in comparison to poly(vinylidene fluoride-co-hexafluoropropene) (PVDF) as reference material, which is established as coating material of DES in clinical applications. Our results show that adhesion could be improved for HUVEC on PDC, PPDO and PEI compared to PVDF, whereas almost no SMC attached to the scaffolds indicating a cell-specific response of HUVEC towards the different fibrous structures. Proliferation and apoptosis results revealed that PPDO and PEI have no significant negative influence on vitality and cell cycle behaviour compared to PVDF. Hence, they represent promising candidates for temporary blood vessel support that induce HUVEC attachment and prevent SMC proliferation.
- Subjects :
- medicine.medical_specialty
Cell Survival
Polymers
Physiology
Polyesters
Myocytes, Smooth Muscle
Polyurethanes
Inflammation
Umbilical vein
Dioxanes
Physiology (medical)
Cell Adhesion
Human Umbilical Vein Endothelial Cells
medicine
Humans
Myocyte
Cell adhesion
Cells, Cultured
Cell Proliferation
Tissue Scaffolds
Chemistry
Cell growth
Hematology
Adhesion
Surgery
medicine.anatomical_structure
Apoptosis
cardiovascular system
Biophysics
Polyvinyls
Stents
medicine.symptom
Cardiology and Cardiovascular Medicine
Cell Division
Blood vessel
Subjects
Details
- ISSN :
- 13860291
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Clinical Hemorheology and Microcirculation
- Accession number :
- edsair.doi.dedup.....16a2e5fcad79207f3884e071a0edd2d8