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Purification of Early and Late Endosomes
- Source :
- Cold Spring Harbor Protocols. 2015:pdb.top074443
- Publication Year :
- 2015
- Publisher :
- Cold Spring Harbor Laboratory, 2015.
-
Abstract
- Proteomic analysis of early and late endosomes has been constrained by the limited purity of the endosomal fractions that can be achieved by biochemical methods. Here we briefly review endocytic pathways, and then introduce fractionation strategies that have been used to improve the purity of isolated endosomes. In addition, we describe innovative proteomics analysis methods that have been shown to partially circumvent the limitations found in the enrichment steps.
Details
- ISSN :
- 15596095 and 19403402
- Volume :
- 2015
- Database :
- OpenAIRE
- Journal :
- Cold Spring Harbor Protocols
- Accession number :
- edsair.doi.dedup.....169b05ab40159f33d9de4b4dc64f7442