Additional file 3 of An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis

Additional file 3: Figure S2. Standard curve for determination of emm75 GE in eNats™ by qPCR. Seven-point standard curves were included on each emm75 qPCR plate and used to extrapolate bacterial load for eNat™ throat swabs from the CHIVAS-M75 trial, expressed as emm75 genome equivalents (GE) per ml of eNat™ medium. The dotted line represents the limit of detection Ct ≤ 35. Mean and standard deviation shown from 7 duplicate runs.