Back to Search
Start Over
Two fluorogenic substrates for purine nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme
- Source :
- Analytical Biochemistry. 446:25-27
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Two nontypical nucleosides, 7-β- d -ribosyl-2,6-diamino-8-azapurine and 8-β- d -ribosyl-2,6-diamino-8-azapurine, have been found to exhibit moderately good, and selective, substrate properties toward calf and bacterial ( Escherichia coli ) forms of purine nucleoside phosphorylase (PNP). The former compound is effectively phosphorolysed by calf PNP and the latter by PNP from E. coli . Both compounds are fluorescent with λ max ∼ 425 to 430 nm, but the reaction product, 2,6-diamino-8-azapurine, emits in a different spectral region ( λ max ∼ 363 nm) with nearly 40% yield, providing a strong fluorogenic effect at 350 to 360 nm.
- Subjects :
- Stereochemistry
Biophysics
Purine nucleoside phosphorylase
medicine.disease_cause
Biochemistry
Substrate Specificity
Escherichia coli
medicine
Animals
Molecular Biology
Fluorescent Dyes
chemistry.chemical_classification
Moderately good
Nucleoside analogue
Substrate (chemistry)
Cell Biology
Fluorescence
Kinetics
Spectrometry, Fluorescence
Enzyme
Purine-Nucleoside Phosphorylase
chemistry
Purines
fluorogenic substrates
8-azapurines
nucleoside analogs
purine-nucleoside phosphorylase
Yield (chemistry)
Cats
medicine.drug
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 446
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....16716033743617191b7bbb80a9b32b99