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Baicalein protects tert-butyl hydroperoxide-induced hepatotoxicity dependent of reactive oxygen species removal
- Source :
- Molecular Medicine Reports. 16:8392-8398
- Publication Year :
- 2017
- Publisher :
- Spandidos Publications, 2017.
-
Abstract
- Baicalein (BA), one of the major bioactive flavonoids isolated from Scutellariae Radix, possesses various pharmacological activities. The present study aimed to investigate the protective effects of BA on tert‑butyl hydroperoxide (t‑BHP)‑induced hepatotoxicity, and to investigate the potential mechanisms in LO2 cells. BA was demonstrated to possess protective properties against t‑BHP injury in LO2 cells, as evidenced by MTT and lactate dehydrogenase assays. BA significantly prevented t‑BHP‑induced depolarization of mitochondrial membrane potential (MMP), decreased the percentage of apoptotic cells caused by t‑BHP, and prevented intracellular reactive oxygen species (ROS) generation in LO2 cells. Furthermore, BA slightly triggered autophagy in LO2 cells, as evidenced by the elevation of LC3‑II expression, while BA combined treatment with an autophagy inhibitor (chloroquine) or activator (rapamycin) did not alter the hepatoprotective properties. In conclusion, BA may possess a hepatoprotective effect against t‑BHP‑induced liver cell injury, dependent on ROS removal. Therefore, BA may represent a potential drug candidate in protecting hepatotoxicity.
- Subjects :
- 0301 basic medicine
Cancer Research
Apoptosis
Biology
Pharmacology
Protective Agents
Biochemistry
Cell Line
03 medical and health sciences
chemistry.chemical_compound
tert-Butylhydroperoxide
Lactate dehydrogenase
Autophagy
Genetics
Humans
Molecular Biology
Membrane Potential, Mitochondrial
chemistry.chemical_classification
Reactive oxygen species
Liver cell
Depolarization
Baicalein
Oxidative Stress
030104 developmental biology
Oncology
chemistry
Flavanones
Hepatocytes
tert-Butyl hydroperoxide
Molecular Medicine
Reactive Oxygen Species
Biomarkers
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....166ab01da4f720f345902b16cfd9f64b