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Pharmacogenetic excitation of the median raphe region affects social and depressive-like behavior and core body temperature in male mice
- Source :
- Life Sciences, Life Sciences, Elsevier, 2021, 286, pp.1-10. ⟨10.1016/j.lfs.2021.120037⟩
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- International audience; AimsMedian raphe region (MRR) is an important bottom-up regulatory center for various behaviors as well as vegetative functions, but detailed descriptions and links between the two are still largely unexplored.MethodsPharmacogenetics was used to study the role of MRR in social (sociability, social interaction, resident intruder test) and emotional behavior (forced swim test) parallel with some vegetative changes (biotelemetry: core body temperature). Additionally, to validate pharmacogenetics, the effect of clozapine-N-oxide (CNO), the ligand of the artificial receptor, was studied by measuring (i) serum and brainstem concentrations of CNO and clozapine; (ii) MRR stimulation induced neurotransmitter release in hippocampus; (iii) CNO induced changes in body temperature and locomotor activity.Key findingsMRR stimulation decreased locomotion, increased friendly social behavior in the resident intruder test and enhanced depressive-like behavior. The latter was accompanied by diminished decrease in core body temperature. Thirty minutes after CNO injection clozapine was predominant in the brainstem. Nonetheless, peripheral CNO injection was able to induce glutamate release in the hippocampus. CNO had no immediate (
- Subjects :
- Male
medicine.medical_specialty
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
biotelemetry
Hippocampus
Stimulation
General Biochemistry, Genetics and Molecular Biology
Body Temperature
social behavior
Mice
median raphe
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
[SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
medicine
Animals
General Pharmacology, Toxicology and Pharmaceutics
Neurotransmitter
Receptor
Clozapine
midbrain raphe
030304 developmental biology
core body temperature
0303 health sciences
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
Depression
Chemistry
[SCCO.NEUR]Cognitive science/Neuroscience
Glutamate receptor
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
General Medicine
depressive-like behavior
Mice, Inbred C57BL
locomotion
Endocrinology
Pharmacogenetics
Raphe Nuclei
clozapine-N-oxide
Brainstem
030217 neurology & neurosurgery
medicine.drug
Behavioural despair test
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 286
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....1667b2af4da3a16caa47881bab7934b9