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Biochemical and Molecular Characterization of the Rice Chalcone Isomerase Family
- Source :
- Plants, Volume 10, Issue 10, Plants, Vol 10, Iss 2064, p 2064 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Chalcone isomerase (CHI) is a key enzyme in flavonoid biosynthesis. In plants, CHIs occur in multigene families, and they are divided into four types, types I–IV. Type I and II CHIs are bona fide CHIs with CHI activity, and type III and IV CHIs are non-catalytic members with different functions. Rice contains seven CHI family genes (OsCHIs). Molecular analysis suggested that OsCHI3 is a type I CHI, and the other OsCHIs were classified into types III and IV. To elucidate their biochemical functions, OsCHI1, OsCHI3, OsCHI6, and OsCHI7 were expressed in Escherichia coli, and the recombinant OsCHI proteins were purified. An activity assay of recombinant OsCHIs showed that OsCHI3 catalyzed the isomerization of naringenin chalcone and isoliquiritigenin, whereas the other recombinant OsCHIs had no CHI activity. OsCHI3 also exhibited a strong preference to naringenin chalcone compared to isoliquiritigenin, which agrees well with the catalytic properties of type I CHIs. These results ascertain OsCHI3 to be a bona fide CHI in rice. OsCHI3 and the other OsCHIs were expressed constitutively throughout the rice growth period and different tissues. OsCHI3 expression was induced immediately in response to ultra-violet (UV) stress, suggesting its involvement in the biosynthesis of sakuranetin, a flavonoid phytoalexin in rice.
- Subjects :
- Chalcone isomerase
sakuranetin
Plant Science
medicine.disease_cause
Article
Sakuranetin
chemistry.chemical_compound
medicine
chalcone isomerase
Naringenin chalcone
Escherichia coli
Ecology, Evolution, Behavior and Systematics
chemistry.chemical_classification
phytoalexin
Ecology
OsCHI family
Phytoalexin
rice
Botany
food and beverages
Enzyme
Flavonoid biosynthesis
Biochemistry
chemistry
QK1-989
Isoliquiritigenin
Subjects
Details
- Language :
- English
- ISSN :
- 22237747
- Database :
- OpenAIRE
- Journal :
- Plants
- Accession number :
- edsair.doi.dedup.....165a2acf464a57fe1fa8da582fedfabf
- Full Text :
- https://doi.org/10.3390/plants10102064