Does intermittent fasting associated with aerobic training influence parameters related to the gut-brain axis of Wistar rats?

Background Intermittent fasting (IF) and aerobic training have demonstrated beneficial effects on intestinal microbiota composition, but little is known about benefits to the brain through the gut-brain axis. The present study aimed to evaluate gut-brain axis parameters in Wistar rats submitted to IF associated or not with aerobic training. Methods Male rats were evaluated for training performance and then randomized into 4 groups of ten: sedentary control (SC), trained control (TC), sedentary intermittent fasting (SIF), and trained intermittent fasting (TIF), and evaluated during four weeks. Results The adiposity index was similar among the TC (2.15±0.43%), SIF (1.98±0.69%) and TIF (1.86±0.51%) groups, and differed from SC (2.98±0.80%). TIF had lower counts of lactic acid bacteria, while SIF had higher counts of Bifidobacterium and Enterococcus. TIF had the highest amount of formic acid in faeces (44.44±2.40 μmol/g) and lowest amount of succinic acid in the gut (0.38±0.00 μmol/g), while SIF had the highest propionic acid amount in the faeces (802.80±00.33 μmol/g) and the lowest amount of lactic acid in the gut (0.85±0.00 μmol/g). TIF demonstrated a tendency towards an anxiolytic effect and SIF showed potential antidepressant effect. IF caused different brain and intestinal injuries. TIF rats presented a diffuse and intense marking of IL-1β in the hippocampus. Conclusion IF and aerobic exercise, associated or not, can modulate parameters related to the gut-brain axis of Wistar rats, and some benefits may be related to the amounts of organic acids.