Back to Search
Start Over
Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception
- Source :
- Europe PubMed Central
- Publication Year :
- 1994
- Publisher :
- The Company of Biologists, 1994.
-
Abstract
- Mammalian skeletal muscle is generated by two waves of fiber formation, resulting in primary and secondary fibers. These fibers mature to give rise to several classes of adult muscle fibers with distinct contractile properties. Here we describe fast myosin heavy chain (MyHC) isoforms that are expressed in nascent secondary, but not primary, fibers in the early development of rat and human muscle. These fast MyHCs are distinct from previously described embryonic and neonatal fast MyHCs. To identify these MyHCs, monoclonal antibodies were used whose specificity was determined in western blots of MyHCs on denaturing gels and reactivity with muscle tissue at various stages of development. To facilitate a comparison of our results with those of others obtained using different antibodies or species, we have identified cDNAs that encode the epitopes recognized by our antibodies wherever possible. The results suggest that epitopes characteristic of adult fast MyHCs are expressed very early in muscle fiber development and distinguish newly formed secondary fibers from primary fibers. This marker of secondary fibers, which is detectable at the time of their inception, should prove useful in future studies of the derivation of primary and secondary fibers in mammalian muscle development.
- Subjects :
- Fetal Proteins
Gene isoform
Muscle tissue
DNA, Complementary
medicine.drug_class
Recombinant Fusion Proteins
Gestational Age
macromolecular substances
Myosins
Biology
Muscle Development
Monoclonal antibody
Epitope
Epitopes
Mice
Myosin
medicine
Animals
Humans
Fiber
Rats, Wistar
Mammals
Muscles
Antibodies, Monoclonal
Gene Expression Regulation, Developmental
Skeletal muscle
Cell Biology
Rats
Blot
medicine.anatomical_structure
Animals, Newborn
Biochemistry
Biomarkers
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....164825512d8e6038afc35d60b4324305