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Effect of dermatan sulphate on a C57-mouse model of pulmonary fibrosis
- Source :
- Journal of International Medical Research, Vol 47 (2019), The Journal of International Medical Research
- Publication Year :
- 2019
- Publisher :
- SAGE Publications, 2019.
-
Abstract
- ObjectiveTo test the antifibrotic effect of dermatan sulphate in a bleomycin-induced mouse model of pulmonary fibrosis.MethodsC57 mice were randomly divided into four experimental groups: saline-treated control group, bleomycin-induced fibrosis group, prednisolone acetate group and dermatan sulphate group. Lungs were assessed using the lung index, and the extent of interstitial fibrosis was graded using histopathological observation of haematoxylin & eosin-stained lung tissue. Lung tissue hydroxyproline levels and blood fibrinogen levels were measured using a hydroxyproline colorimetric kit and the Clauss fibrinogen assay, respectively. Tissue-type plasminogen activator (tPA) was measured using a chromogenic tPA assay kit.ResultsLung index values were significantly lower in the dermatan sulphate group versus the fibrosis group. Histopathological analyses revealed that dermatan sulphate treatment ameliorated the increased inflammatory cell infiltration, and attenuated the reduction in interstitial thickening, associated with bleomycin-induced fibrosis. Hydroxyproline and fibrinogen levels were decreased in the dermatan sulphate group versus the fibrosis model group. Dermatan sulphate treatment was associated with increased tPA levels versus controls and the fibrosis group.ConclusionsDamage associated with bleomycin-induced pulmonary fibrosis was alleviated by dermatan sulphate.
- Subjects :
- Male
0301 basic medicine
Medicine (General)
Pathology
medicine.medical_specialty
Dermatan Sulfate
Bleomycin
Biochemistry
Mice
03 medical and health sciences
chemistry.chemical_compound
R5-920
0302 clinical medicine
Pulmonary fibrosis
medicine
Animals
Antibiotics, Antineoplastic
pulmonary fibrosis
bleomycin
business.industry
Dermatan sulphate
Biochemistry (medical)
Anticoagulants
Cell Biology
General Medicine
respiratory system
Pre-Clinical Research Reports
medicine.disease
Mice, Inbred C57BL
carbohydrates (lipids)
Disease Models, Animal
Hydroxyproline
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Female
business
Subjects
Details
- ISSN :
- 14732300 and 03000605
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Journal of International Medical Research
- Accession number :
- edsair.doi.dedup.....163a0f516e04bfcfee410c0080fe36ea