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Telomere shortening in the colonic mucosa of patients with ulcerative colitis
- Source :
- Journal of Gastroenterology. 33:343-348
- Publication Year :
- 1998
- Publisher :
- Springer Science and Business Media LLC, 1998.
-
Abstract
- Telomere length in human somatic cells gradually decreases with the number of cell divisions and is regarded as a marker of somatic cell turnover. Mucosal cells of the affected colon show rapid turnover in individuals with active ulcerative colitis (UC). Telomere length was determined by Southern blot analysis of terminal restriction fragments (TRFs) from the colonic mucosa of 17 patients with UC in remission, two of whom showed dysplasia, and 17 control subjects without colitis. For each individual, mean TRF length was compared between rectal mucosa and unaffected cecal mucosa. The mean TRF length of the rectal mucosa was significantly less than that of cecal mucosa in UC patients (7.87 +/- 0.36kb versus 8.77 +/- 0.21 kb; P = 0.0015, Wilcoxon signed rank test), whereas no significant difference was detected in the control subjects. The extent of telomere shortening was 10.6 +/- 3.35% in UC patients, compared with 0.8 +/- 0.64% in noncolitis controls (P = 0.0024, Mann-Whitney U-test). Four UC patients, two of whom had dysplasia, showed telomere shortening of more than 20% in the rectal mucosa. These observations suggest that telomere shortening in the colonic mucosa of individuals with UC may represent the history of mucosal inflammation during disease of long duration, and that it may contribute to aneuploidy in UC.
- Subjects :
- Adult
Male
medicine.medical_specialty
Pathology
Adolescent
Somatic cell
Cell
Aneuploidy
Biology
Gastroenterology
Internal medicine
medicine
Humans
Intestinal Mucosa
Colitis
Aged
Middle Aged
Telomere
Hepatology
medicine.disease
Ulcerative colitis
Blotting, Southern
medicine.anatomical_structure
Dysplasia
Colitis, Ulcerative
Female
Subjects
Details
- ISSN :
- 14355922 and 09441174
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....16345ef1ae8f91863019998e27de749a