Additional file 4: of Bimodal dynamics of primary metabolism-related responses in tolerant potato-Potato virus Y interaction

Validation of microarray results by RT-qPCR. Microarray results were validated by analyzing eight biologically relevant genes involved in photosynthesis (chlorophyll a-b binding protein: CAB and RuBisCO activase: RA), defense response (β-1,3-glucanase of three classes: Glu-I, Glu-II, Glu-III and pathogenesis-related protein1b: PR-1b), and sugar metabolism (granule bound starch synthase I: GBSSI and CwINV: cell wall invertase) by quantitative real-time PCR. Expression values were log2 transformed, and a fold-change difference (log2FC) was calculated for PVYNTN versus mock in cv. Désirée and NahG-Désirée at 1, 3, 4, 5 and 7 dpi are shown in the table. Statistically significant values (p