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Rapid analysis of allelic variants of the sheep PrP gene by oligonucleotide probes
- Source :
- Microbiology and immunology. 42(8)
- Publication Year :
- 1998
-
Abstract
- A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with 32P-labeled nine allele-specific oligonucleotide probes corresponding to the polymorphic PrP codons 112, 136, 154 and 171. Three allelic variants of the PrP gene, PrP(MARQ), PrP(TARQ) and PrP(MARR), were found in the flocks. Among those variants, nearly half of the ewes had alleles of the 171-Arg variant that is closely associated with resistance to natural scrapie. Assessments of allelic mutations of the PrP gene may help to select the scrapie-resistant progenitors in the flocks.
- Subjects :
- Male
PrPSc Proteins
animal diseases
Immunology
DNA Mutational Analysis
Scrapie
Biology
medicine.disease_cause
Microbiology
Polymerase Chain Reaction
chemistry.chemical_compound
Virology
Genotype
medicine
Animals
Allele
Polymerase
Alleles
Genetics
Mutation
Polymorphism, Genetic
Sheep
Oligonucleotide
Genetic Variation
Nucleic Acid Hybridization
Molecular biology
chemistry
biology.protein
Female
Flock
Disease Susceptibility
Oligonucleotide Probes
DNA
Subjects
Details
- ISSN :
- 03855600
- Volume :
- 42
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Microbiology and immunology
- Accession number :
- edsair.doi.dedup.....162511aac539ac00ecc800e61c5bf793