Complete separation of phospholipids from human heart combining two HPLC methods

Authors :: G. Rocquelin
Pierre Juanéda
Unité mixte de recherche nutrition lipidique et régulation fonctionnelle du coeur et des vaisseaux
Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)
Source :: Lipids, Lipids, Springer Verlag, 1986, 21 (3), pp.239-240
Publication Year :: 1986
Publisher :: HAL CCSD, 1986.
Abstract: The separation of phospholipid classes from human heart was achieved in two steps by high performance liquid chromatography (HPLC) using a silica column with an ultraviolet spectromonitor at 206 nm. A complete partitioning of phosphatidylcholines (PC), phosphatidylethanolamines (PE), phosphatidylinositols (PI), phosphatidylserines (PS), cardiolipins (CL), lysophosphatidylcholines (LPC) and sphingomyelins (Sph) was obtained for further analysis.

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