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Secretoneurin, an Angiogenic Neuropeptide, Induces Postnatal Vasculogenesis

Authors :
Marcy Silver
Allison Hanley
Toshinori Murayama
Ewald Woell
Michael T. Pedrini
Douglas W. Losordo
Marianne Brodmann
Wolfgang Sturm
Josef R. Patsch
Dirk H. Walter
Margot Egger
Markus Nagl
Rudolf Kirchmair
Peter Schratzberger
Silke Frauscher
Reiner Fischer-Colbrie
Andreas Niederwanger
Wolfgang Eisterer
Source :
Circulation. 110:1121-1127
Publication Year :
2004
Publisher :
Ovid Technologies (Wolters Kluwer Health), 2004.

Abstract

Background—Induction of postnatal vasculogenesis, the mobilization of bone marrow–derived endothelial progenitor cells and incorporation of these cells into sites of blood vessel formation, is a well-known feature of angiogenic cytokines such as vascular endothelial growth factor. We hypothesized that the angiogenic neuropeptide secretoneurin induces this kind of neovascularization.Methods and Results—Secretoneurin induced mobilization of endothelial progenitor cells to sites of vasculogenesis in vivo in the cornea neovascularization assay. Progenitor cells were incorporated into vascular structures or were located adjacent to them. Systemic injection of secretoneurin led to increase of circulating stem cells and endothelial progenitor cells. In vitro secretoneurin induced migration, exerted antiapoptotic effects, and increased the number of these cells. Furthermore, secretoneurin stimulated the mitogen-activated protein kinase system, as shown by phosphorylation of extracellular signal–regulated kinase, and activated the protein kinase B/Akt pathway. Activation of mitogen-activated protein kinase was necessary for increase of cell number and migration, whereas Akt seemed to play a role in migration of endothelial progenitor cells.Conclusions—These data show that the angiogenic neuropeptide secretoneurin stimulates postnatal vasculogenesis by mobilization, migration, and incorporation of endothelial progenitor cells.

Details

ISSN :
15244539 and 00097322
Volume :
110
Database :
OpenAIRE
Journal :
Circulation
Accession number :
edsair.doi.dedup.....1619db18f510db4908d886d8700a3477
Full Text :
https://doi.org/10.1161/01.cir.0000139884.81390.56