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Regulation of Fasciclin II and Synaptic Terminal Development by the Splicing Factor Beag
- Source :
- The Journal of Neuroscience. 32:7058-7073
- Publication Year :
- 2012
- Publisher :
- Society for Neuroscience, 2012.
-
Abstract
- Pre-mRNA alternative splicing is an important mechanism for the generation of synaptic protein diversity, but few factors governing this process have been identified. From a screen forDrosophilamutants with aberrant synaptic development, we identifiedbeag, a mutant with fewer synaptic boutons and decreased neurotransmitter release.Beagencodes a spliceosomal protein similar to splicing factors in humans andCaenorhabditis elegans. We find that bothbeagmutants and mutants of an interacting genedsmu1have changes in the synaptic levels of specific splice isoforms of Fasciclin II (FasII), theDrosophilaortholog of neural cell adhesion molecule. We show that restoration of one splice isoform of FasII can rescue synaptic morphology inbeagmutants while expression of other isoforms cannot. We further demonstrate that this FasII isoform has unique functions in synaptic development independent of transsynaptic adhesion.beaganddsmu1mutants demonstrate an essential role for these previously uncharacterized splicing factors in the regulation of synapse development and function.
- Subjects :
- Gene isoform
Genetics
Spliceosome
biology
Cell Adhesion Molecules, Neuronal
General Neuroscience
Alternative splicing
Neuromuscular Junction
Presynaptic Terminals
biology.organism_classification
Article
Cell biology
Animals, Genetically Modified
Alternative Splicing
Splicing factor
Mutation
RNA splicing
Spliceosomes
Animals
Drosophila Proteins
Protein Isoforms
Drosophila
Neural cell adhesion molecule
Caenorhabditis elegans
Drosophila Protein
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....1606fc0a335d67c21028912d9a6bf454
- Full Text :
- https://doi.org/10.1523/jneurosci.3717-11.2012