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Isolation and Characterisation of a High-Efficiency Desaturase and Elongases from Microalgae for Transgenic LC-PUFA Production

Authors :
Stan S. Robert
Susan I. Blackburn
Anne Maree Mackenzie
Maged P. Mansour
Qing Liu
Dion Matthew Frederick Frampton
Pushkar Shrestha
Peter D. Nichols
James Robertson Petrie
Surinder P. Singh
Source :
Marine Biotechnology. 12:430-438
Publication Year :
2009
Publisher :
Springer Science and Business Media LLC, 2009.

Abstract

The production of long-chain polyunsaturated fatty acids from precursor molecules linoleic acid (LA; 18:2omega6) and alpha-linolenic acid (ALA; 18:3omega3) is catalysed by sequential desaturase and elongase reactions. We report the isolation of a front-end Delta6-desaturase gene from the microalgae Ostreococcus lucimarinus and two elongase genes, a Delta6-elongase and a Delta5-elongase, from the microalga Pyramimonas cordata. These enzymes efficiently convert their respective substrates when transformed in yeast (39-75% conversion for omega3 substrate fatty acids), and the Delta5-elongase in particular displays higher elongation efficiency (75% for conversion of eicosapentaenoic acid (20:5omega3) to docosapentaenoic acid (22:5omega3)) than previously reported genes. In addition, the Delta6-desaturase is homologous with acyl-CoA desaturases and shows a strong preference for the omega3 substrate ALA.

Details

ISSN :
14362236 and 14362228
Volume :
12
Database :
OpenAIRE
Journal :
Marine Biotechnology
Accession number :
edsair.doi.dedup.....160456f29e55f0792bed13664b8cc905
Full Text :
https://doi.org/10.1007/s10126-009-9230-1