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Isolation and Characterisation of a High-Efficiency Desaturase and Elongases from Microalgae for Transgenic LC-PUFA Production
- Source :
- Marine Biotechnology. 12:430-438
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- The production of long-chain polyunsaturated fatty acids from precursor molecules linoleic acid (LA; 18:2omega6) and alpha-linolenic acid (ALA; 18:3omega3) is catalysed by sequential desaturase and elongase reactions. We report the isolation of a front-end Delta6-desaturase gene from the microalgae Ostreococcus lucimarinus and two elongase genes, a Delta6-elongase and a Delta5-elongase, from the microalga Pyramimonas cordata. These enzymes efficiently convert their respective substrates when transformed in yeast (39-75% conversion for omega3 substrate fatty acids), and the Delta5-elongase in particular displays higher elongation efficiency (75% for conversion of eicosapentaenoic acid (20:5omega3) to docosapentaenoic acid (22:5omega3)) than previously reported genes. In addition, the Delta6-desaturase is homologous with acyl-CoA desaturases and shows a strong preference for the omega3 substrate ALA.
- Subjects :
- Fatty Acid Desaturases
Linolenic acid
Linoleic acid
Molecular Sequence Data
Saccharomyces cerevisiae
Biology
Applied Microbiology and Biotechnology
chemistry.chemical_compound
Acetyltransferases
Chlorophyta
Microalgae
Amino Acid Sequence
Transgenes
Phylogeny
Plant Proteins
chemistry.chemical_classification
Substrate (chemistry)
Eicosapentaenoic acid
Yeast
Enzyme
chemistry
Biochemistry
Fatty Acids, Unsaturated
Docosapentaenoic acid
Sequence Alignment
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 14362236 and 14362228
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Marine Biotechnology
- Accession number :
- edsair.doi.dedup.....160456f29e55f0792bed13664b8cc905
- Full Text :
- https://doi.org/10.1007/s10126-009-9230-1