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Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor Posidonia meadows health state
- Source :
- BMC Ecology, Vol 7, Iss 1, p 6 (2007), BMC Ecology
- Publisher :
- Springer Nature
-
Abstract
- Background The endemic seagrass Posidonia oceanica (L.) Delile colonizes soft bottoms producing highly productive meadows that play a crucial role in coastal ecosystems dynamics. Human activities and natural events are responsible for a widespread meadows regression; to date the identification of "diagnostic" tools to monitor conservation status is a critical issue. In this study the feasibility of a novel tool to evaluate ecological impacts on Posidonia meadows has been tested. Quantification of a putative stress indicator, i.e. phenols content, has been coupled to 2-D electrophoretic protein analysis of rhizome samples. Results The overall expression pattern from Posidonia rhizome was determined using a preliminary proteomic approach, 437 protein spots were characterized by pI and molecular weight. We found that protein expression differs in samples belonging to sites with high or low phenols: 22 unique protein spots are peculiar of "low phenols" and 27 other spots characterize "high phenols" samples. Conclusion Posidonia showed phenols variations within the meadow, that probably reflect the heterogeneity of environmental pressures. In addition, comparison of the 2-D electrophoresis patterns allowed to highlight qualitative protein expression differences in response to these pressures. These differences may account for changes in metabolic/physiological pathways as adaptation to stress. A combined approach, based on phenols content determination and 2-D electrophoresis protein pattern, seems a promising tool to monitor Posidonia meadows health state.
- Subjects :
- Proteomics
Posidonia
Settore BIO/07
two dimensional gel electrophoresis
chemistry
biosynthesi
Alismatidae
Phenols
Environmental Science(all)
Botany
Electrophoresis, Gel, Two-Dimensional
Ecosystem
proteomic
QH540-549.5
Ecology, Evolution, Behavior and Systematics
Plant Proteins
General Environmental Science
growth, development and aging
Alismatales
Two-dimensional gel electrophoresis
Ecology
biology
article
biology.organism_classification
Rhizome
Seagrass
Posidonia oceanica
Research Article
Environmental Monitoring
Subjects
Details
- Language :
- English
- ISSN :
- 14726785
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Ecology
- Accession number :
- edsair.doi.dedup.....15f4438399141bf77bc15ecaeb4fba18
- Full Text :
- https://doi.org/10.1186/1472-6785-7-6