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A Broadly Cross-protective Vaccine Presenting the Neighboring Epitopes within the VP1 GH Loop and VP2 EF Loop of Enterovirus 71
- Source :
- Scientific Reports
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are the major etiological agents of hand, foot and mouth disease (HFMD) and are often associated with neurological complications. Currently, several vaccine types are being developed for EV71 and CA16. In this study, we constructed a bivalent chimeric virus-like particle (VLP) presenting the VP1 (aa208-222) and VP2 (aa141-155) epitopes of EV71 using hepatitis B virus core protein (HBc) as a carrier, designated HBc-E1/2. Immunization with the chimeric VLPs HBc-E1/2 induced higher IgG titers and neutralization titers against EV71 and CA16 in vitro than immunization with only one epitope incorporated into HBc. Importantly, passive immunization with the recombinant HBc-E2 particles protected neonatal mice against lethal EV71 and CA16 infections. We demonstrate that anti-VP2 (aa141-155) sera bound authentic CA16 viral particles, whereas anti-VP1 (aa208-222) sera could not. Moreover, the anti-VP2 (aa141-155) antibodies inhibited the binding of human serum to virions, which demonstrated that the VP2 epitope is immunodominant between EV71 and CA16. These results illustrated that the chimeric VLP HBc-E1/2 is a promising candidate for a broad-spectrum HFMD vaccine and also reveals mechanisms of protection by the neighboring linear epitopes of the VP1 GH and VP2 EF loops.
- Subjects :
- viruses
Amino Acid Motifs
Cross Reactions
Antibodies, Viral
Article
Epitope
Neutralization
law.invention
Epitopes
law
Enterovirus 71
Animals
Rats, Wistar
Mice, Inbred BALB C
Multidisciplinary
biology
Viral Vaccine
Vaccination
Virion
virus diseases
Viral Vaccines
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Antibodies, Neutralizing
Virology
Enterovirus A, Human
Titer
biology.protein
Recombinant DNA
Capsid Proteins
Female
Antibody
Hand, Foot and Mouth Disease
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....15e4d0ac1c89bedaa30f34d5942dd659