INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023

Aim. The investigation of the influence of some physicochemical factors on dehydrogenase activity of Bacillus subtilis IMB B-7023. Methods. The bacteria were cultivated in the periodic process. Dehydrogenase activity was evaluated on reduction of 2,3,5-tetrazolium chloride to triphenilphormazane, the concentration of which was determined by photocolorymetrical methods. Results. It was defined that dehydrogenase activity reached the greatest significance in the given strain at the temperature of 37 °C in the media having pH 7.0. The highest indices of dehydrogenase activity have been obtained in the content of 10 g/l glucose in it. It was determined that ions of Mg2+ and Ca2+ have stimulated dehydrogenase activity of Bacillus subtilis, and ions of Fe3+, on the contrary, suppressed it. Сonclusion. Actual presence of high sensitiveness of the dehydrogenase complex of these bacteria to physicochemical factors of the environment can be used for making up the prognoses of the given strain activity under certain factors of the environment in its use as a complex bacterial preparation in agroecosystems.