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Improved apparatus for the differential measurement of pH: applications to the measurement of glucose
- Source :
- Analytical biochemistry. 112(2)
- Publication Year :
- 1981
-
Abstract
- Two improved systems for the determination of the difference in pH between two solutions at the same temperature are described, both of which have a long-term stability of ±1 × 10 −4 pH unit and a peak-to-peak noise level of 5 × 10 −5 pH unit when operated in the differential mode. The cells are constructed of Delrin which has been found to be inert in biological fluids. These cells, with a volume of 1 ml, can be electrically connected to each other via a dialysis membrane and are thermostated during the duration of the pH measurements to ±0.01°C. In a second configuration, combination microelectrodes are inserted into each cell; thus the dialysis membrane is not necessary. To demonstrate the application of these systems to reactions of biological interest where pH changes occur, we investigated the hexokinase-catalyzed reaction between ATP and glucose in a phosphate buffer. The concentrations of glucose determined from the observed changes in pH by a numerical solution to the equilibrium equations are in excellent agreement with the analytical values.
- Subjects :
- Inert
Chemical Phenomena
Chemistry
Biophysics
Analytical chemistry
Cell Biology
Equilibrium equation
Hydrogen-Ion Concentration
Ph changes
Biochemistry
Differential measurement
Dialysis tubing
Phosphates
Solutions
Microelectrode
Adenosine Triphosphate
Glucose
Volume (thermodynamics)
Hexokinase
Electrode
Methods
Molecular Biology
Electrodes
Microelectrodes
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 112
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Analytical biochemistry
- Accession number :
- edsair.doi.dedup.....15dff3559332f530ac0b009d500ac8e6