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The Homologous ABI5 and EEL Transcription Factors Function Antagonistically to Fine-Tune Gene Expression during Late Embryogenesis
- Source :
- The Plant cell, The Plant cell, American Society of Plant Biologists (ASPB), 2002, 14 (6), pp.1391-403. ⟨10.1105/tpc.000869⟩, Plant Cell, Plant Cell, American Society of Plant Biologists, 2002, 14 (6), pp.1391-403. ⟨10.1105/tpc.000869⟩
- Publication Year :
- 2002
- Publisher :
- Oxford University Press (OUP), 2002.
-
Abstract
- In Arabidopsis, the basic leucine zipper transcription factor ABI5 activates several late embryogenesis-abundant genes, including AtEm1 and AtEm6. However, the expression of many other seed maturation genes is independent of ABI5. We investigated the possibility that ABI5 homologs also participate in the regulation of gene expression during seed maturation. We identified 13 ABI5-related genes in the Arabidopsis genomic sequence. RNA gel blot analysis showed that seven of these genes are active during seed maturation and that they display distinct expression kinetics. We isolated and characterized two mutant alleles of one of these genes, AtbZIP12/EEL. Unlike abi5, the eel mutations did not inhibit the expression of any of the maturation marker genes that we monitored. On the contrary, the accumulation of the AtEm1 and AtEm6 mRNAs was enhanced in eel mutant seeds compared with wild-type seeds. Gel mobility shift assays, combined with analysis of the genetic interactions among the eel and abi5 mutations, indicated that ABI5 and EEL compete for the same binding sites within the AtEm1 promoter. This study illustrates how two homologous transcription factors can play antagonistic roles to fine-tune gene expression.
- Subjects :
- 0106 biological sciences
Mutant
Arabidopsis
MESH: Amino Acid Sequence
Plant Science
MESH: Base Sequence
Polymerase Chain Reaction
01 natural sciences
Gene Expression Regulation, Plant
MESH: Gene Expression Regulation, Developmental
Gene expression
MESH: Genes, Plant
Developmental
MESH: Arabidopsis
Promoter Regions, Genetic
MESH: Phylogeny
Phylogeny
Plant Proteins
Genetics
Regulation of gene expression
0303 health sciences
MESH: Plant Proteins
biology
Gene Expression Regulation, Developmental
MESH: Transcription Factors
MESH: Leucine Zippers
MESH: Promoter Regions (Genetics)
Basic-Leucine Zipper Transcription Factors
Multigene Family
Seeds
Promoter Regions (Genetics)
Research Article
MESH: DNA Primers
Leucine zipper
Molecular Sequence Data
MESH: Sequence Alignment
MESH: Arabidopsis Proteins
MESH: Basic-Leucine Zipper Transcription Factors
Genes, Plant
03 medical and health sciences
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Amino Acid Sequence
MESH: Gene Expression Regulation, Plant
Transcription factor
Gene
DNA Primers
030304 developmental biology
Leucine Zippers
ATF3
MESH: Molecular Sequence Data
Base Sequence
Arabidopsis Proteins
MESH: Polymerase Chain Reaction
Plant
Cell Biology
biology.organism_classification
Gene Expression Regulation
Genes
MESH: Seeds
MESH: Multigene Family
Sequence Alignment
Transcription Factors
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1532298X and 10404651
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- The Plant Cell
- Accession number :
- edsair.doi.dedup.....15c7158bbfa52ef0f8e6acfe185bc5e6