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Synthesis of Silica-Coated Magnetic Nanoparticles and Application in the Detection of Pathogenic Viruses
- Source :
- Journal of Nanomaterials, Vol 2013 (2013)
- Publication Year :
- 2013
- Publisher :
- Hindawi Limited, 2013.
-
Abstract
- Magnetic Fe3O4nanoparticles were prepared by coprecipitation and then coated with silica. These Fe3O4/SiO2nanoparticles consisted of a 10–15 nm magnetic core and a silica shell of 2–5 nm thickness. The superparamagnetic property of the Fe3O4/SiO2particles with the magnetization of 42.5 emu/g was confirmed by vibrating sample magnetometer (VSM). We further optimized buffers with these Fe3O4/SiO2nanoparticles to isolate genomic DNA of hepatitis virus type B (HBV) and of Epstein-Barr virus (EBV) for detection of the viruses based on polymerase chain reaction (PCR) amplification of a 434 bp fragment ofSgene specific for HBV and 250 bp fragment of nuclear antigen encoding gene specific for EBV. The purification efficiency of DNA of both HBV and EBV using obtained Fe3O4/SiO2nanoparticles was superior to that obtained with commercialized Fe3O4/SiO2microparticles, as indicated by (i) brighter PCR-amplified bands for both HBV and EBV and (ii) higher sensitivity in PCR-based detection of EBV load (copies/mL). The time required for DNA isolation using Fe3O4/SiO2nanoparticles was significantly reduced as the particles were attracted to magnets more quickly (15–20 s) than the commercialized microparticles (2-3 min).
- Subjects :
- Materials science
Article Subject
Coprecipitation
Nanoparticle
Molecular biology
DNA extraction
law.invention
chemistry.chemical_compound
Magnetization
chemistry
law
lcsh:Technology (General)
Magnetic nanoparticles
lcsh:T1-995
General Materials Science
DNA
Polymerase chain reaction
Superparamagnetism
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 16874129 and 16874110
- Volume :
- 2013
- Database :
- OpenAIRE
- Journal :
- Journal of Nanomaterials
- Accession number :
- edsair.doi.dedup.....1591ca089e9e9a1fce4c851d1198e14c