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Paper-thin multilayer microfluidic devices with integrated valves
- Source :
- Lab on a chip. 21(7)
- Publication Year :
- 2021
-
Abstract
- Integrated valve microfluidics has an unparalleled capability to automate the rapid delivery of fluids at the nanoliter scale for high-throughput biological experimentation. However, multilayer soft lithography, which is used to fabricate valve-microfluidics, produces devices with a minimum thickness of around five millimeters. This form-factor limitation prevents the use of such devices in experiments with limited sample thickness tolerance such as 4-pi microscopy, stimulated Raman scattering microscopy, and many forms of optical or magnetic tweezer applications. We present a new generation of integrated valve microfluidic devices that are less than 300 μm thick, including the cover-glass substrate, that resolves the thickness limitation. This "thin-chip" was fabricated through a novel soft-lithography technique that produces on-chip micro-valves with the same functionality and reliability of traditional thick valve-microfluidic devices despite the orders of magnitude reduction in thickness. We demonstrated the advantage of using our thin-chip over traditional thick devices to automate fluid control while imaging on a high-resolution inverted microscope. First, we demonstrate that the thin-chip provides improved signal to noise when imaging single cells with two-color stimulated Raman scattering (SRS). We then demonstrated how the thin-chip can be used to simultaneously perform on-chip magnetic manipulation of beads and fluorescent imaging. This study reveals the potential of our thin-chip in high-resolution imaging, sorting, and bead capture-based single-cell multi-omics applications.
- Subjects :
- Materials science
Orders of magnitude (temperature)
Microfluidics
Biomedical Engineering
Bioengineering
02 engineering and technology
Substrate (electronics)
Biochemistry
03 medical and health sciences
symbols.namesake
Reliability (semiconductor)
Multilayer soft lithography
Lab-On-A-Chip Devices
Microscopy
030304 developmental biology
0303 health sciences
business.industry
Inverted microscope
Reproducibility of Results
General Chemistry
021001 nanoscience & nanotechnology
symbols
Optoelectronics
Glass
0210 nano-technology
business
Raman scattering
Subjects
Details
- ISSN :
- 14730189
- Volume :
- 21
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Lab on a chip
- Accession number :
- edsair.doi.dedup.....158b22c90a955b65a9f5019c11f549c6