Back to Search
Start Over
Bioplastic (poly-3-hydroxybutyrate) production by the marine bacterium Pseudodonghicola xiamenensis through date syrup valorization and structural assessment of the biopolymer
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Biobased degradable plastics have received significant attention owing to their potential application as a green alternative to synthetic plastics. A dye-based procedure was used to screen poly-3-hydroxybutyrate (PHB)-producing marine bacteria isolated from the Red Sea, Saudi Arabia. Among the 56 bacterial isolates, Pseudodonghicola xiamenensis, identified using 16S rRNA gene analyses, accumulated the highest amount of PHB. The highest PHB production by P. xiamenensis was achieved after 96 h of incubation at pH 7.5 and 35 °C in the presence of 4% NaCl, and peptone was the preferred nitrogen source. The use of date syrup at 4% (w/v) resulted in a PHB concentration of 15.54 g/L and a PHB yield of 38.85% of the date syrup, with a productivity rate of 0.162 g/L/h, which could substantially improve the production cost. Structural assessment of the bioplastic by Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy revealed the presence of methyl, hydroxyl, methine, methylene, and ester carbonyl groups in the extracted polymer. The derivative products of butanoic acid estimated by gas chromatography-mass spectrometry [butanoic acid, 2-amino-4-(methylseleno), hexanoic acid, 4-methyl-, methyl ester, and hexanedioic acid, monomethyl ester] confirmed the structure of PHB. The present results are the first report on the production of a bioplastic by P. xiamenensis, suggesting that Red Sea habitats are a potential biological reservoir for novel bioplastic-producing bacteria.
- Subjects :
- 0301 basic medicine
Geologic Sediments
Hydroxybutyrates
lcsh:Medicine
010501 environmental sciences
Sodium Chloride
01 natural sciences
Bioplastic
Ribotyping
chemistry.chemical_compound
Marine bacteriophage
Biopolymers
Spectroscopy, Fourier Transform Infrared
Food science
Rhodobacteraceae
lcsh:Science
Indian Ocean
Phylogeny
Hexanoic acid
Multidisciplinary
biology
Phoeniceae
Nuclear magnetic resonance spectroscopy
Water Microbiology
Biotechnology
Polyesters
Industrial Waste
Biodegradable Plastics
engineering.material
Microbiology
Gas Chromatography-Mass Spectrometry
Article
03 medical and health sciences
Industrial Microbiology
Seawater
Fourier transform infrared spectroscopy
Nuclear Magnetic Resonance, Biomolecular
0105 earth and related environmental sciences
Bacteriological Techniques
lcsh:R
biology.organism_classification
Culture Media
030104 developmental biology
chemistry
Yield (chemistry)
engineering
lcsh:Q
Biopolymer
Plant Preparations
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....158982b50c06add846e9a5e05211fe8c