Genome-wide Identification, Characterization and Expression profile of TALE gene family in (Juglans regia L.)

Background The three amino acid loop extension (TALE) are essential transcription factors that regulate the differentiation of meristem in the plant growth and development. there's a lot of journalism of the TALE family in plants, such as Arabidopsis, rice, and others. however, there's no report on the TALE family in walnut. Results In this study, the genome-wide identification and expression analysis of the TALE gene family were studied comprehensively in walnut. Based on the specific structure of the TALE domain, a total of twenty-seven walnut TALE genes (JrTALE) were identified. According to different domains structure and phylogenetic relationships, the JrTALE gene family could be divided into two subfamilies, namely BELL and KNOX. This family was relatively conservative in evolution. Exon-intron structure analysis showed that there were 3 to 6 exons, and they had a wide range of exons length. Analysis of protein conserved motifs showed that JrTALE contained similar motif structures in the same phylogenetic branch. Expression pattern analysis of the TALE family genes in walnut showed that JrTALE41 and JrTALE42 were differentially expressed in female and male flower buds. We confirmed that JrTALE4-5, JrTALE8-12, JrTALE36-37, JrTALE46-53, and JrTALE14-15 were differentially expressed between different stages of flower bud development by real-time quantitative PCR (qRT-PCR). Conclusions Sixty-nine JrTALEs were identified in the genome-wide. the physicochemical properties, phylogenetic relationships, gene structure, promoter cis-acting elements, and protein interactions were systematically analyzed by bioinformatics methods. This study provides valuable information about the TALE family in the transitional development of walnut flower buds, which lay the foundation for further understanding of TALE family genes and in walnut.