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Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis
- Source :
- Biochemical and Biophysical Research Communications. 391:1687-1692
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- A novel alpha-iso-cubebenol, which has anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, was isolated from the fruits of Schisandra chinensis. alpha-iso-cubebenolinhibited LPS-induced nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production. Consistent with these findings, alpha-iso-cubebenol also reduced the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 at the protein and mRNA levels in a concentration-dependent manner. alpha-iso-cubebenol also inhibited LPS-induced nuclear translocation of the NF-kappaB p65 subunit. Furthermore, alpha-iso-cubebenol suppressed the phosphorylation of ERK, JNK, and p38 kinase induced by LPS. Since the novel alpha-iso-cubebenol blocked the production of several pro-inflammatory mediators induced by LPS in macrophages, the molecule can be useful material for the development of anti-inflammatory agents against bacterial infections or endotoxin.
- Subjects :
- Lipopolysaccharides
MAPK/ERK pathway
Lipopolysaccharide
Schisandra chinensis
p38 mitogen-activated protein kinases
medicine.medical_treatment
Biophysics
Nitric Oxide Synthase Type II
Biochemistry
Cell Line
Nitric oxide
Mice
chemistry.chemical_compound
medicine
Animals
Enzyme Inhibitors
Molecular Biology
Schisandra
Mitogen-Activated Protein Kinase Kinases
Cyclooxygenase 2 Inhibitors
biology
Terpenes
Macrophages
Anti-Inflammatory Agents, Non-Steroidal
NF-kappa B
NF-κB
Cell Biology
biology.organism_classification
Molecular biology
Nitric oxide synthase
chemistry
Cyclooxygenase 2
biology.protein
lipids (amino acids, peptides, and proteins)
Sesquiterpenes
Prostaglandin E
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 391
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....15826ac61704fb88dcb134fabf60d60d