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Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases
- Source :
- Angewandte Chemie. 126:6852-6855
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- 5-[(2-Nitrobenzyl)oxymethyl]-2′-deoxyuridine 5′-O-triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5-hydroxymethyluracil-modified nucleic acids, which were fully recognized and cleaved by restriction enzymes.
- Subjects :
- DNA clamp
biology
DNA polymerase
Ultraviolet Rays
DNA polymerase II
General Chemistry
DNA
DNA Restriction Enzymes
DNA-Directed DNA Polymerase
General Medicine
Molecular biology
Catalysis
Restriction fragment
Pentoxyl
chemistry.chemical_compound
Restriction enzyme
chemistry
Biochemistry
biology.protein
Flap endonuclease
Polymerase
Subjects
Details
- ISSN :
- 00448249
- Volume :
- 126
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie
- Accession number :
- edsair.doi.dedup.....15803e9df7439bae040c02dac9fdcc1a