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Differential distribution of calpain small subunit 1 and 2 in rat brain
- Source :
- European Journal of Neuroscience. 19:1819-1825
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- Calpains, the Ca(2+)-dependent thiol proteases, are abundant in the nervous tissue. The ubiquitous enzyme forms in mammals are heterodimers consisting of a specific, micro or m, large (catalytic) subunit and, apparently, a common small (regulatory) subunit (CSS1). Recently, however, we described a second form of small subunit (CSS2), which is of restricted occurrence [Schad, E., Farkas, A., Jekely, G., Tompa, P. & Friedrich, P. (2002) Biochem. J., 362, 383-388]. Here we analysed the distribution of immunoreactivity in various parts of rat brain against two anti-CSS1 and two anti-CSS2 antibodies by correlated light and electron microscopy. Remarkably, the antibodies showed differential distribution in various parts of rat cortex: anti-CSS1 reacted mainly with perikarya and dendrites, whereas anti-CSS2 was more prominent in axons. In serial sections CSS2 and synaptophysin gave very similar patterns, i.e. these epitopes seem to colocalize. Electron microscopy confirmed that CSS1 was mainly localized postsynaptically in dendrites and somata, whereas CSS2 was found presynaptically. The hypothesis is advanced that these distinct distributions of calpain subunits may be related to the transport of these enzymes in nerve cells.
- Subjects :
- Male
Protein subunit
Synaptophysin
Epitope
Rats, Sprague-Dawley
Sequence Analysis, Protein
Calpain small subunit 1
medicine
Animals
Humans
Axon
Microscopy, Immunoelectron
biology
Calpain
General Neuroscience
Nervous tissue
Brain
Colocalization
Immunohistochemistry
Molecular biology
Rats
Protein Subunits
medicine.anatomical_structure
biology.protein
Subjects
Details
- ISSN :
- 14609568 and 0953816X
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- European Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....1578bf5f97d915c9fb226d9885bae90e