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Molecular chaperone alphaB-crystallin is expressed in the human fetal telencephalon at midgestation by a subset of progenitor cells
- Source :
- Journal of neuropathology and experimental neurology. 69(7)
- Publication Year :
- 2010
-
Abstract
- Alphab-crystallin (CRYAB) is a small heat shock protein with a chaperoning activity that is present in the postnatal healthy human brain in oligodendrocytes and in a few astrocytes. The involvement of CRYAB in cell differentiation, proliferation, signaling, cytoskeletal assembly, and apoptosis in various model systems has suggested that it might also play a role in the developing human brain. We analyzed the distribution and the levels of this molecular chaperone in healthy and polygenetically compromised (Down syndrome [DS]) human telencephalon at midgestation. We demonstrate that CRYAB is expressed in a temporospatial pattern by numerous radial glial cells and some early oligodendrocyte progenitors, including dividing cells, as well as a few astroglial cells in both healthy and DS fetal brains. We also found abundant phosphorylation of CRYAB at Ser-59, which mediates its antiapoptotic and cytoskeletal functions. There was only marginal phosphorylation at Ser-45.In contrast to our earlier study in young DS subjects, upregulation of phosphorylated CRYAB occurred rarely in DS fetuses. The distribution, the timing of appearance, and the results of colocalization studies suggest that CRYAB assists in the biological processes associated with developmental remodeling/differentiation and proliferation of select subpopulations of progenitor cells in human fetal brain at midgestation.
- Subjects :
- Telencephalon
Gestational Age
Nerve Tissue Proteins
Biology
Pathology and Forensic Medicine
Cellular and Molecular Neuroscience
Fetus
Downregulation and upregulation
Crystallin
Heat shock protein
medicine
Serine
Humans
Progenitor cell
Phosphorylation
Embryonic Stem Cells
Age Factors
Colocalization
Gene Expression Regulation, Developmental
alpha-Crystallin B Chain
General Medicine
Human brain
Oligodendrocyte
Cell biology
medicine.anatomical_structure
Neurology
Neuroglia
Neurology (clinical)
Down Syndrome
Neuroscience
Subjects
Details
- ISSN :
- 15546578
- Volume :
- 69
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of neuropathology and experimental neurology
- Accession number :
- edsair.doi.dedup.....1568d0b4eaf8f8530fcb03e085417f44