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Icariin derivative inhibits inflammation through suppression of p38 mitogen-activated protein kinase and nuclear factor-kappaB pathways
- Source :
- Biologicalpharmaceutical bulletin. 33(8)
- Publication Year :
- 2010
-
Abstract
- In this study we investigated the anti-inflammatory effects of an icariin derivative (3,5-dihydroxy-4'-methoxy-6'',6''-dimethy1-4'',5''-dihydropyrano[2'',3'':7,8]-flavone). We found that this icariin derivative inhibits tumor necrosis factor-alpha (TNF-alpha) production, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression, and protein expression in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages. It also alleviates paw edema induced by carrageenan in mice. To clarify the molecular mechanisms underlying these anti-inflammatory effects, we examined the effects of this compound on the phosphorylation of mitogen-activated protein kinase (MAPK), phosphorylation of inhibitory kappaBalpha (IkappaBalpha), and nuclear translocation of p65 subunit of nuclear factor (NF)-kappaB, and found it suppresses the activation of p38 MAPK and inhibits translocation of NF-kappaB p65 to the nucleus through decreasing the phosphorylation of IkappaBalpha. As a result of these properties, this icariin derivative can be considered as a potential drug for inflammatory diseases.
- Subjects :
- MAPK/ERK pathway
Lipopolysaccharides
Cell Survival
p38 mitogen-activated protein kinases
Blotting, Western
Cell Culture Techniques
Pharmaceutical Science
Nitric Oxide Synthase Type II
p38 Mitogen-Activated Protein Kinases
Cell Line
chemistry.chemical_compound
Mice
Animals
Edema
Protein kinase A
Pharmacology
Cell Nucleus
Flavonoids
biology
Molecular Structure
Chemistry
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Anti-Inflammatory Agents, Non-Steroidal
NF-kappa B
Transcription Factor RelA
General Medicine
Flavones
Cell biology
Nitric oxide synthase
Protein Transport
Biochemistry
Cyclooxygenase 2
Mitogen-activated protein kinase
biology.protein
Phosphorylation
Tumor necrosis factor alpha
Icariin
Subjects
Details
- ISSN :
- 13475215
- Volume :
- 33
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Biologicalpharmaceutical bulletin
- Accession number :
- edsair.doi.dedup.....1556ac25df04983c3c2b40d3ffd33260