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Shear stress induced nuclear shrinkage through activation of Piezo1 channels in epithelial cells
- Source :
- Journal of Cell Science.
- Publication Year :
- 2019
- Publisher :
- The Company of Biologists, 2019.
-
Abstract
- The cell nucleus responds to mechanical cues with changes in size, morphology and motility. Previous work has shown that external forces couple to nuclei through the cytoskeleton network, but we show here that changes in nuclear shape can be driven solely by calcium levels. Fluid shear stress applied to MDCK cells caused the nuclei to shrink through a Ca2+-dependent signaling pathway. Inhibiting mechanosensitive Piezo1 channels through treatment with GsMTx4 prevented nuclear shrinkage. Piezo1 knockdown also significantly reduced the nuclear shrinkage. Activation of Piezo1 with the agonist Yoda1 caused similar nucleus shrinkage in cells not exposed to shear stress. These results demonstrate that the Piezo1 channel is a key element for transmitting shear force input to nuclei. To ascertain the relative contribution of Ca2+ to cytoskeleton perturbation, we examined F-actin reorganization under shear stress and static conditions, and showed that reorganization of the cytoskeleton is not necessary for nuclear shrinkage. These results emphasize the role of the mechanosensitive channels as primary transducers in force transmission to the nucleus.
- Subjects :
- Cell Nucleus Shape
Shear force
Biology
Mechanotransduction, Cellular
Ion Channels
Cell Line
Madin Darby Canine Kidney Cells
03 medical and health sciences
Dogs
0302 clinical medicine
medicine
Shear stress
Animals
Calcium Signaling
Cytoskeleton
030304 developmental biology
Shrinkage
Cell Nucleus
0303 health sciences
PIEZO1
Epithelial Cells
Cell Biology
Cell nucleus
medicine.anatomical_structure
Biophysics
Calcium
Mechanosensitive channels
Stress, Mechanical
Nucleus
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....15525ba1670d7bc5c22979a2a885e4e9