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Replication and Immunogenicity of Swine, Equine, and Avian H3 Subtype Influenza Viruses in Mice and Ferrets
- Source :
- Journal of Virology. 87:6901-6910
- Publication Year :
- 2013
- Publisher :
- American Society for Microbiology, 2013.
-
Abstract
- Since it is difficult to predict which influenza virus subtype will cause an influenza pandemic, it is important to prepare influenza virus vaccines against different subtypes and evaluate the safety and immunogenicity of candidate vaccines in preclinical and clinical studies prior to a pandemic. In addition to infecting humans, H3 influenza viruses commonly infect pigs, horses, and avian species. We selected 11 swine, equine, and avian H3 influenza viruses and evaluated their kinetics of replication and ability to induce a broadly cross-reactive antibody response in mice and ferrets. The swine and equine viruses replicated well in the upper respiratory tract of mice. With the exception of one avian virus that replicated poorly in the lower respiratory tract, all of the viruses replicated in mouse lungs. In ferrets, all of the viruses replicated well in the upper respiratory tract, but the equine viruses replicated poorly in the lungs. Extrapulmonary spread was not observed in either mice or ferrets. No single virus elicited antibodies that cross-reacted with viruses from all three animal sources. Avian and equine H3 viruses elicited broadly cross-reactive antibodies against heterologous viruses isolated from the same or other species, but the swine viruses did not. We selected an equine and an avian H3 influenza virus for further development as vaccines.
- Subjects :
- Swine
viruses
Immunology
Cross Reactions
Biology
Antibodies, Viral
Virus Replication
medicine.disease_cause
Microbiology
H5N1 genetic structure
Virus
Birds
Mice
Orthomyxoviridae Infections
Neutralization Tests
Virology
Vaccines and Antiviral Agents
Pandemic
Influenza A virus
medicine
Animals
Horses
Mice, Inbred BALB C
Immunogenicity
Ferrets
Antibodies, Neutralizing
medicine.anatomical_structure
Viral replication
Insect Science
biology.protein
Female
Antibody
Respiratory tract
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....15451c26224ee0c9f1eaeb46e3c33dfd
- Full Text :
- https://doi.org/10.1128/jvi.03520-12